Denatured globin body detection

Denatured globin corpuscle detection (Heinz body) is a denatured hemoglobin granule, usually attached to the cell membrane, which occurs in sensitive individuals after taking drugs or exposure to chemicals, which can cause Hb denaturation. Others can be seen in patients with unstable Hb, which is valuable for the diagnosis of G-6-PD deficiency and unstable Hb disease. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Do not tamper with venous blood collection, wait patiently for results. Normal value Red blood cells containing 5 or more globin bodies in normal people are generally less than 30%. Clinical significance Abnormal result The test results were positive, that is, the red blood cells containing 5 or more globin bodies were more than 30%. G-6-PD deficiency is often higher than 45%, so it can be used as a screening test for G-6-PD deficiency. However, reduced glutathione deficiency is also increased; the percentage of cells containing small bodies in unstable hemoglobin disease is 75% to 84%, and is also increased in HbH disease and chemical poisoning. People who need to be examined are suspected of having G-6-PD deficiency. Precautions Inappropriate crowd: None. Taboo before the test: pay attention to rest, keep blood on an empty stomach. Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood draw, causing blood vessels in the arms. Avoid strenuous exercise. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. The blood samples were added to the acetophenone and incubated at 37 ° C for 2 to 4 hours. The formation of globin bodies in the red blood cells was observed by staining with tar tar blue, and the percentage of red blood cells containing 5 or more globin bodies was calculated. Not suitable for the crowd No taboos. Adverse reactions and risks 1, dizziness or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 2. Risk of infection: If you use an unclean needle, you may be at risk of infection.

The material in this site is intended to be of general informational use and is not intended to constitute medical advice, probable diagnosis, or recommended treatments.