kaolin partial thromboplastin time

At 37 °C, kaolin can activate factors XI and XII, and partial thromboplastin, cephalin, can replace platelet factor 3 and accelerate the activation of factor X. KPTT measures the time required for plasma coagulation under the condition of adding enough kaolin and partial thromboplastin and calcium ions. This test is a screening test for endogenous coagulation system, but lacks specificity. Further tests should be performed to identify the type of coagulopathy. It is meaningful to extend KPTT by more than 10 s compared with normal controls. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: It is best to use coagulation instrument and supporting kit. Normal value Male 37 ± 3.3 (31.5 ~ 43.5) s. Female 37.5 ± 2.8 (32 ~ 43) s. Clinical significance Abnormal result KPTT is more meaningful than normal (35 ~ 45s) control for more than 10s. This test is sensitive to clotting time. When various factors involved in plasma thromboplastin production are lacking, KPTT can be prolonged. Especially, the hemophilia caused by the decrease of VIII, IX and XI factors is prolonged. Light hemophilia with factor VIII: C < 25% was detected. Prothrombin, fibrinogen is severely reduced, and prolonged in the presence of anticoagulant substances. It can also be used as one of the indicators in the treatment of hemophilia and anticoagulant drugs. KPTT shortening is seen in DIC hypercoagulable phase and thrombocytopenia. Low results may be diseases: neonatal scleredema, high incidence of essential thrombocytosis may be diseases: hemophilia, diffuse intravascular coagulation considerations (1) Specimens should be inspected in time, no more than 2 hours at the latest. The plasma should be separated at 3000 r/min to remove platelets. The activation time of plasma plus kaolin partial thromboplastin should not be less than 3 min. (2) The quality of white clay can affect the measurement results. If the normal control is prolonged, it indicates that the suspension of the thrombin partial thromboplastin is of poor quality. Inspection process (1) Take 0.1 ml/L sodium oxalate anticoagulant 0.2 ml, add venous blood 1.8 ml, and centrifuge to separate plasma. (2) Take 0.1 ml of plasma, add 0.1 ml of white cousin partial thromboplastin suspension, shake well, and incubate for 3 min in a 37 ° C water bath (between gently). (3) Add 0.1ml of 0.025mol/LCaCl2 solution, immediately start the stopwatch, oscillate continuously in the water bath, and observe carefully. When the fibrin wire appears, the recording time is KPTT value. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.

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