α2-plasmin inhibitory antigen

The α2 plasmin inhibitor is mainly synthesized by the liver, a single-chain glycoprotein, which is a serine protease with specific inhibitory activity in vivo. It inhibits the action of plasmin in a limited time and inhibits the binding of plasminogen to fibrin to prevent fiber. The protein is hydrolyzed by antiplasmin. Hyperlipidemia, obesity, oral contraceptives, coronary heart disease, myocardial infarction, arterial thrombosis, ischemic stroke and other t-PA levels are reduced. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Before the examination, the diet is light, alcohol is forbidden, and the morning is needed to check for an empty stomach to ensure a good night's sleep. Normal value 1 to 12 ng/ml. Clinical significance (1) The content of t-PA increased with age, strenuous exercise and stress response, and venous retention led to an increase in t-PA content. (2) Congenital t-PA content increase. (3) Reduction of t-PA content such as hyperlipidemia, obesity, oral contraceptives, coronary heart disease, myocardial infarction, arterial thrombosis, and ischemic stroke. Low results may be diseases: coronary heart disease, obesity, high blood lipids ELISA is a specific and sensitive immunological test. Inspection process (1) The t-PA antibody was diluted with 0.05 mol/L carbonate buffer. (2) Dilute the antibody-POD conjugate with a dilution buffer. (3) The o-phenylenediamine (8 mg/ml) was dissolved in a matrix buffer, and 10 μl of 30% H 2 O 2 was added. (4) The t-PA standard was diluted to 10, 5, 2.5, 1.25, 0.625, and 0.3125 IU/ml. (5) Specimen dilution: 1 part of sodium citrate anticoagulated plasma plus 5 parts of dilution. If the t-PA value is estimated to increase, the plasma is diluted 1:10. (6) The ELISA plate was coated with diluted t-PA antibody, 200 μl per well, incubated overnight, and then washed 3 times with washing solution. (7) Add standard/sample 200 μl, and after 1 h of incubation, wash 3 times as above. (8) Add 200 μl of peroxidase antibody complex, and after 1 h of incubation, wash the same as above for 3 times, and add the matrix immediately after washing. (9) Add 200 μl of matrix to each well and develop for 10-15 min. (10) Add 50 μl of sulfuric acid (3 mol/L) or 100 μl of hydrochloric acid (1 mol/L), stop the reaction for 10 min, colorimetrically at 492 nm, within 2 h, with a dilution buffer as the base. (11) The t-PA content was found from the standard curve according to the absorbance reading. Not suitable for the crowd It is not advisable for pregnant and lactating women to perform this test. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.

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