serotonin

5-HT is also known as serotonin, which is derived from tryptophan. Tryptophan is reacted with tryptophan hydroxylase to form 5-hydroxytryptophan, and then decarboxylase becomes 5-HT. 5-HT is a delivery substance of the central nervous system, and its active part is guanamine. It is widely found in brain, platelets, stomach and other tissues, with the largest content in the brain, and is a strong smooth muscle stimulation and vasoconstrictor. It is high in platelets and participates in vasoconstriction after release of platelets. 2/3 of 5-HT is discharged after the liver is combined with sulfuric acid or glucuronic acid, or is broken down by decomposition of strontium; about 1/3 is oxidatively deaminated by monoamine oxidase to form 5-HLAA and then excreted from the urine. 5-HIAA is the final product of 5-hydroxytryptophan metabolism and is not biologically active. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: Keep a normal mindset. Normal value The serum was 0.22 to 2.06 μmol/L (39 to 361 ng/ml). Clinical significance Abnormal result (1) elevated in carcinoid tumor syndrome, postoperative dumping syndrome, migraine, hypoxia and so on. (2) reduce connective tissue diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (rheumatic arthritis), mixed connective tissue disease, Parkinson's disease (tremor palsy), chorea, liver disease Nuclear degeneration (Wilson's disease), schizophrenia, neurasthenia, etc. High results may be diseases: elderly Parkinson's disease, pregnancy with thrombotic disease, vasoactive intestinal peptide tumor, essential thrombocytosis, phenylketonuria in children It can also rise after eating a lot of walnuts and bananas. Inspection process (1) Membrane receptor preparation: 1 brain homogenate: rat or mouse decapitated blood, cut the head into the ice bath, and use the bone scissors to cut the skull immediately remove the brain, remove the capsule and residual blood, cut and add a considerable weight of 20 times the volume The pre-cooled solution 1 was homogenized at 10,000 r/min with an in-line high-speed disperser for 1 min, and then homogenized by an ultrasonic pulverizer for 60 s. The whole process was carried out in an ice bath. 2 Centrifugation: The brain homogenate was centrifuged at 1000×g for 10 min to remove the nucleus and dense substance. The supernatant was carefully transferred to another centrifuge tube with a dropper, centrifuged at 50,000×g for 10 min, and the supernatant was discarded. The precipitate was washed twice with solution 1 and centrifuged twice. The resulting precipitate is a cell membrane fragment containing receptors. All operations were carried out at 0 to 4 °C. 3 Suspension: The precipitate was suspended in solution 2 and homogenized by an ultrasonic pulverizer for 60 s. Then, solution 2 was used to prepare 11.76 mg of the wet weight of brain tissue per ml of the suspension. Take 0.2ml and determine its protein content by lowry method. Put it in the ice bath. (2) Binding reaction: The total volume of the reaction was 2 ml, and the test tube was divided into two parts, one part was the total binding reaction tube, and the other part was non-specifically bound to the reaction tube. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks May cause an infection.

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