Hepatitis D Antibodies

Hepatitis DVirus (HDV) is a defective single-stranded negative-strand RNA virus with a diameter of 35-37 nm and a spherical shape. Its outer membrane is HBsAg, and its core contains specific hepatitis D virus antigen (HDAg) and Viral RNA. During the maturation process, HDV needs to be assembled into intact virus particles by means of HBsAg as an outer membrane protein. HDV itself is not infected and can only be infected with HBsAg-positive people. HDVAg is highly antigenic and can stimulate the body to produce IgM and IgG antibodies. These antibodies are non-neutralizing antibodies and have no protective effect on the body. HDV laboratory detection can be detected by nucleic acid hybridization and PCR methods, and HDVAg, anti-HDVIgM, and anti-HDV total antibodies can also be detected by ELISA and RIA. Basic Information Specialist classification: Infectious disease examination and classification: liver function examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: Positive with hepatitis D virus. Tips: Repeat the test for suspected specimens. Normal value no. Clinical significance It can be determined whether or not there is hepatitis D virus. Positive result may be disease: D-type viral hepatitis precautions (1) Because hepatitis D and hepatitis B infection exist at the same time, after peripheral blood is treated with detergent, HBAg and HBcAg may exist at the same time. Therefore, attention should be paid to prevent interference of HBcAg. Therefore, non-labeled substances must be added to the enzyme label. Monoclonal antibodies that specifically neutralize HBcAg are excluded. (2) Because the amount of antigen in the peripheral blood is small, the serum should not be diluted at least 30 ~ 50μl. (3) It is best to use the colorimetric method to avoid false positives or false negatives. (4) Repeat the test for suspected specimens. Inspection process Follow the kit instruction manual. 1. Add sample: Add 50 μl of sample to each well of the coated reaction plate, set the negative and positive control for each test, and keep at 37 ° C for 1 h, and wash the plate 3 times. 2. Add enzyme combination: Add 50 μl of anti-HDV-HRP to each well, incubate at 37 ° C for 1 h, and wash the plate 5 times. 3. Color development: 100 μl of the substrate solution was added to each well, and after incubation at room temperature for 15 min, 50 μl of the stop solution was added to each well. 4. Interpretation of the results: On the microplate reader, the reagent blank is used to check the zero point, and the optical density value of each hole at 492 nm is measured. The judgment value is first calculated. When the sample A value is higher than the judgment value, the value is lower than the judgment value. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.

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