Rotavirus antibody (RV Ab)

Rotavirus is the most important virus for acute diarrhea in animals and humans. There are currently 7 groups of rotaviruses reported (A to G). Rotavirus antibody assays are used to diagnose diseases of rotavirus infection. Rotavirus mainly affects infants and young children aged 2-6 years, causing acute diarrhea; chronic infection is rare, mainly in immunodeficient children and immunosuppressed patients after bone marrow transplantation; the symptoms of adult rotavirus infection are mild. Basic Information Specialist classification: Digestive examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: abnormal. Tips: Pay attention to food hygiene. Normal value negative. Clinical significance Rotavirus antibody assays are used to diagnose diseases of rotavirus infection. Rotavirus mainly affects infants and young children aged 2-6 years, causing acute diarrhea; chronic infection is rare, mainly in immunodeficient children and immunosuppressed patients after bone marrow transplantation; the symptoms of adult rotavirus infection are mild. Positive results may be diseases: diarrhea in children, acute diarrhea, precautions for acquired immunodeficiency syndrome The enzyme-linked immunosorbent assay was used, and the specimen was fecal and immediately sent for examination. Inspection process Operation method: (1) The optimal concentration of rabbit anti-rotavirus antibody was coated as single test well, and the normal rabbit serum was coated with double wells as control well, 100μl per well, 4°C for 24h, washed 3 times, and stored at 4°C after drying. spare. (2) The stool to be tested is diluted with 0.01mol/L PBS into a 20% suspension, centrifuged at 3000r/min for 30min, and the supernatant is taken, and an equal amount of diluent (containing 20.0g/L bovine serum albumin) is added, 100μl per well. Each sample was added with 2 wells of test and control wells. Each plate was set to 1 each of a positive (viral antigen) and a negative (normal stool) control. Wash overnight at 4 ° C for 3 times. (3) Dilute the guinea pig anti-rotavirus antibody to the optimal concentration, add 100 μl per well, wash at 37 ° C for 1 h, and wash 3 times. (4) Add enzyme-labeled goat anti-guinea pig IgG, 100 μl per well, 37 ° C for 1 h, and wash 3 times. (5) Add o-phenylenediamine-H2O2 substrate solution, 100 μl per well, at 37 ° C for 20 min. The reaction was stopped by adding 2 mol/L of H 2 SO 4 , and the absorbance at 492 nm was measured by an ELISA detector. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.

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