Fluorescent Treponema Antibody Adsorption Assay

The Fluorescent Treponema Antibody Absorption Test (FTA-ABS) is an indirect fluorescent antibody method. Before the test, the patient's serum was treated with Reiter's spirochete ultrasonic lysate, and the cross-reactive antibody was removed, and then reacted with Treponema pallidum, and then fluorescein-labeled anti-human IgG was added. This method has high sensitivity and specificity, and can detect the specific antibodies of Treponema pallidum at the earliest. However, the operation is complicated and the patient continues to be positive after the drug treatment. Therefore, it is not suitable for judging the therapeutic effect. For the determination of efficacy, refer to the results of the serotonin test. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: Positive is syphilitic cardiovascular disease. Tips: The sensitivity and specificity of this test are high. Normal value negative. Clinical significance Positive: syphilitic cardiovascular disease. Positive results may be diseases: elderly syphilitic heart disease precautions The sensitivity and specificity of this test are high, and it is first positive in early syphilis and is often used as a confirmatory test. Inspection process (1) The test serum inactivated by a water bath at 56 ° C for 30 min was diluted 1:5 with an absorbent and thoroughly mixed. (2) Add 0.03 ml of non-specific control serum diluted 1:5 with PBS and absorbent, respectively, in the slide reaction loop of the immobilized antigenic spirochete. This is a negative control. (3) Add 0.03 ml of the minimum reactivity (+) diluted with the absorbent to 1:5 to control the serum in the slide reaction loop. This is a positive control. (4) Add 0.03 ml of the test serum diluted 1:5 by the absorbent to the slide reaction circle. (5) The slide was placed in a wet box at 36 ° C ± 1 ° C for 30 min. (6) Place the slide on a slide holder, rinse the slide with PBS (pH 7.2 ± 0.1) for 5 s, and then immerse in the PBS for 5 min. Rinse, immerse the slides and wash for 30 times. (7) Dilute with distilled water that has been sterilized at 120 ° C for 15 min for 5 min. Gently blot the slide with absorbent paper. (8) 0.03 ml of fluorescein isothiocyanate-labeled anti-human IgG (FITC-labeled anti-human IgG) was added to each reaction zone. Repeat steps 5 through 7. (9) Add a small drop of fixative to the slide, then cover the cover slip, and do not form bubbles. (10) Place the slide under a fluorescence microscope, first use a tungsten lamp as the light source, and look for the Nichols spiral in a dark field. Then use a high-pressure mercury lamp and watch the fluorescence results by selecting the UV section through the filter. The reading should be completed within 4 hours. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.

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