colloidal iron staining

Colloidal iron staining means that under pH 2.0, the free acidic group in the cell has affinity for colloidal iron, and then interacts with acidic potassium ferrocyanide. The Prussian blue reaction can occur in the site of the acidic mucopolysaccharide in the cytoplasm. It is displayed in blue. The promyelocytes with increased particles showed a strong positive reaction, and the Auer bodies were also stained dark blue and positive, which is valuable for the diagnosis of acute promyelocytic leukemia. Basic Information Specialist classification: Oncology examination classification: microscopy Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: The promyelocytes with increased particles showed a strong positive reaction, and the Auer bodies were also stained dark blue and positive, which is valuable for the diagnosis of acute promyelocytic leukemia. Tips: After 8 pm on the day before the medical examination, you should fast, so as not to affect the detection of indicators such as blood glucose in the second sky. Normal value The granulocyte-derived granulocytes were negative, and the promyelocytes and the subsequent stages were mostly positive. Monocytes, lymphocytes, plasma cells, and young red blood cells all had negative reactions. Clinical significance Abnormal results: The promyelocytes with increased particles showed a strong positive reaction, and the Auer bodies were also stained dark blue and positive, which is valuable for the diagnosis of acute promyelocytic leukemia. People who need to be tested: the elderly, people who work long hours under radiation. High results may be diseases: acute promyelocytic leukemia, follicular mucin disease considerations Due to the different experimental conditions of each laboratory, the range of reference values ​​is different, which should be based on the reference range of the laboratory. Inspection process (1) The tissue sections were deparaffinized into water and washed with 0.01 mol/l of pH 7.4 PBS. (2) The tissue was blocked with 4% normal sheep serum at 37 ° C for 30 min. (3) The tissues were incubated with the first antibody, respectively, at 4 ° C for 24 h, and PBS was shaken for 10 min × 3. (4) A second antibody labeled with colloidal iron was incubated at 37 ° C for 30 min or with a bridge anti-coupling iron marker. (5) Distilled water wash. (6) Coloring with 20% of a mixture of 1% potassium ferrocyanide and 1% hydrochloric acid. (7) Washing with tap water. (8) Nuclear solid red dyed core, dehydrated, transparent, and sealed. Dyeing by this method, the positive is blue, and the nucleus is red. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.

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