Anti-hepatitis E virus IgM antibody

Hepatitis E virus (HEV) is a pathogen of hepatitis E caused by intestinal transmission. It is spherical, has no cyst on the surface, is stereotacticly icosahedral, has a protruding structure on the surface, and the internal nucleic acid is single-stranded RNA. Hepatic RNA virus genus of the genus Vibrio. HEV has the surface antigen HEVAg, which stimulates the body to produce antibodies. The laboratory test of HEV is mainly to detect antibodies, such as IgA, IgM and IgG. Because IgA and IgM have similar meanings, IgM and IgG are generally detected. The detection method is mainly ELISA. Basic Information Specialist classification: Infectious disease examination and classification: liver function examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Anti-HEVIgM is positive, indicating an acute phase of HEV infection. Tips: It is advisable to have a light diet on the day before the inspection, and work on time to avoid affecting the next day's inspection. Normal value negative. Clinical significance Anti-HEVIgM appeared earlier but lasted for a shorter period of time. Anti-HEVIgM is positive, indicating an acute phase of HEV infection. However, many patients do not have anti-HEVIgM antibodies, so the absence of antibodies does not exclude HEV infection. Positive result may be disease: pre- treatment of hepatitis E virus As to whether anti-HEV is a protective antibody, it tends to have some protection at present, but it is still not clear. Inspection process 1, laboratory materials blood. 2. Principle of determination of anti-hepatitis E virus IgM antibody Indirect ELISA, ie, coating the HEV recombinant antigen on the plate, adding the serum to be tested, adding the enzyme-labeled anti-human IgM, and adding the substrate. If there is anti-HEVIgM in the serum to be tested, the color is positive and vice versa. Does not develop color. 3, reagents Use a commercial kit officially approved by the health department. (The antigen is 2 to 3 polypeptide fragments, and the coating solution is preferably 10 mmol/L pH 7.4 PBS. The sample dilution is pH 7.2 Tris-HCl, 10 g/LBSA, 1 ml/L polysorbate 20. Enzyme-labeled anti-human IgG.) 4, the operation method Operate according to the kit instructions, or as follows: The antigen polypeptide is coated on a microplate, washed at room temperature overnight, 3 times. Add 100 μl of the diluted solution to each well, and make 5 μl of the sample. Set a negative and positive control at the same time, wash at 37 ° C for 30 min, and wash 6 times. 100 μl of HRP anti-human IgG was added to each well, and washed at 37 ° C for 30 min for 6 times. 100 μl of substrate solution (TMB-H2O2) per well was placed at room temperature for 15 min. The reaction was stopped by adding 10 g/LSDS 50 μl, and the 630 nm A value was measured. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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