Starch hydrolysis test

The starch hydrolysis test is an experiment in which starch is hydrolyzed into a monosaccharide. Microorganisms cannot directly utilize the starch of macromolecules, and it is necessary to decompose macromolecular substances by the extracellular enzymes produced to be absorbed and utilized by microorganisms. The extracellular enzyme is mainly a hydrolase, and the substance having a large molecular weight is degraded into a smaller compound by the action of the hydrolase, so that it can be transported into the cell. For example, amylase hydrolyzed starch is a small molecule of dextrin, disaccharide and monosaccharide. The starch encountered an iodine solution that turned blue. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Tips: People who need to check: There are various symptoms caused by bacterial infections caused by various acid-producing gases, such as abdominal fullness, discomfort or pain, often accompanied by other adverse symptoms such as heating, bloating, acid reflux and loss of appetite Wait. Normal value The digestive system and absorption system are in a healthy state. Clinical significance Abnormal results Some bacteria hydrolyze starch and use its hydrolysate to produce acid-producing gas that causes discomfort in the human body. People who need to be examined: There are various symptoms caused by bacterial infections caused by various acid-producing gases, such as abdominal fullness, discomfort or pain, often accompanied by other adverse symptoms such as heating, bloating, acid reflux and loss of appetite. Precautions Starch hydrolyzed intermediate dextrin (having a larger molecular weight red dextrin and a lower molecular weight white dextrin), the color change of iodine reaction is: purple-brown-yellow, if the starch is not completely hydrolyzed, there will be different The color appears. Inspection process Starch can be hydrolyzed under the catalysis of acid; the hydrolysis process of starch: a dextrin with a smaller molecular weight (product of incomplete hydrolysis of starch), dextrin continues to hydrolyze to form maltose, and the final hydrolysate is glucose. Medium: The bacteria were inoculated on a plate, cultured in a 37-degree incubator for 24 hours, and Gram's iodine solution was added to the colony to observe the color change. The blue color was negative and no blue was positive. Not suitable for the crowd no. Adverse reactions and risks no.

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