urinary follicle stimulating hormone

Follicle Stimulating Hormone (FSH) and LH are collectively called gonadotropins, and are the same glycoproteins as TSH. They promote the development of follicles, promote the secretion of estrogen together with LH, cause ovulation, synergize testosterone to promote the formation of testicular tubules and sperm. Formed, pulsed, and women change with menstrual cycle. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Tips: It is forbidden to exercise vigorously, heavy physical labor, stop taking clomiphene, levodopa, birth control pills, sex hormones and other drugs. Women in the menstrual period are not suitable for this examination. Normal value Female follicular phase 5 ~ 20u / 24h; The luteal phase is 5-15u/24h; Ovulation period 15 ~ 16u / 24h; Menstrual period 50 ~ 100u / 24h. Clinical significance (1) elevation: congenital ovarian hypoplasia (gonadal hypoplasia), congenital testicular hypoplasia (sperm tube dysplasia), testicular feminism, gonadectomy, gonadal stimulating hormone tumor, polycystic ovary Syndrome, etc. (2) reduction: anterior pituitary dysfunction, Sheen syndrome (Xihan syndrome), anorexia nervosa, familial olfactory-sexual hypoplasia syndrome (olfactory dysfunction-free testis syndrome), retina Pigment degeneration-obesity-multi-finger syndrome (sexual naive-retinal retinitis-polyphagia, toe deformity syndrome), sex hormone tumor, HCG (human chorionic gonadotropin) tumor, adrenal hyperplasia syndrome, and the like. High results may be diseases: congenital ovarian hypoplasia, testicular feminization considerations Before the test: prohibit strenuous exercise, heavy physical labor, stop taking clomiphene, levodopa, birth control pills, sex hormones and other drugs. When checking: Relax your body and eliminate tension and anxiety. Inspection process Urine FSH extraction: A. acetone precipitation, take 5ml of urine into a conical centrifuge tube, add 1ml of extraction buffer, gently rotate and mix. B. Add 20 ml of cold acetone, cover and mix up and down for more than 10 times. C. Place in the -10 ° C refrigerator overnight or 72h. D. Centrifugation (1500 x g) for 30 min, a little sediment was observed. E. Aspirate the supernatant and dry the pellet with nitrogen (about 30 min). This should be done in a fume hood. F. Add 1 ml of zero standard solution, grind the precipitate with a glass rod, gently shake and repeat several times (about 5 minutes) until the sediment has completely sunk. G. Centrifuge (1500 × g) for 15 min to retain the supernatant (that is, the extracted FSH part), for storage and storage, the FSH content conversion coefficient of the urine sample is 5. Finally, check with a microscope. Not suitable for the crowd Not suitable for the crowd: women in the menstrual period. Adverse reactions and risks No.

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