In vivo platelet activation markers

The exact indication for the detection of platelet activity in vivo has not been found. The current study aims to investigate whether platelet activity is useful for the diagnosis of unstable thoracic aortic aneurysms, stents, and subsequent coronary prosthetic or stroke prognosis or atherosclerosis. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Immediately inhibit cell viability. Normal value β-TG～35μg/L PF-4～15μg/L P-selectin expression (CD62p) ≤ 4% CD63 expression ≤ 4% A special laboratory reference value is required. Clinical significance Increases in plasma β-TG and PF-4 can be seen in the following conditions: prosthetic heart valves, coronary heart disease, acute myocardial infarction, diabetes, peripheral venous occlusion, and pulmonary embolism. In other acute cerebral infarction or other arterial thrombosis, the plasma level is 6 to 10 times higher than that of normal people. This concentration represents the content of β-TG and PF-4 in 1% platelets at normal platelet concentrations. It is suggested that this method may reflect platelet activity due to poor acquisition techniques or improper treatment of blood samples. For example, only 1 in 1000 platelets release their granules, which can lead to a 2-fold increase in plasma levels of β-TG and PF-4. Activation of platelet immunocytological measurements in the circulation suggests an increase in cardiovascular and thrombotic diseases in particular. There is a definite study showing that the number of activated platelets in the circulation is a prognostic factor for early reperfusion after coronary balloon dilatation (PTCA). High results may be diseases: coronary heart disease, acute myocardial infarction A key issue in detecting activated platelets in the body is to prevent human platelet activation during and after blood collection. Artificial platelet activation in the beta-TG, PF-4 method can be reduced by using standard blood sample mixtures and adhering to appropriate blood collection rules. Further, β-TG and PF-4 are metabolized by the kidney, so the plasma concentrations of both are dependent on renal function. There is currently no accepted sample guide to detect activated platelets in circulation. However, the stabilizing effect of mixing directly with formaldehyde after sample collection will meet the following rules: (1) Immediate inhibition of cell viability. (2) No antigen destruction. (3) The shape is stable. (4) There is no spontaneous immunofluorescence in the cells. Inspection process Enzyme-free radioimmunoassay. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.

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