Estrogen

Estrogen is a steroid hormone, mainly composed of ovary, follicle, corpus luteum and placenta. It has biological activity and it is valuable to determine estrogen for gynecological diseases. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Ovarian dysfunction, congenital ovarian hypoplasia (Turne syndrome), Sheehan syndrome (postpartum pituitary dysfunction syndrome), Sinmond syndrome, anorexia nervosa, fetal-placental insufficiency, etc. Normal value: Serum (male): 40-115 Serum (female, cycle 1-10 days): 61-394 Above normal: Excessive ovarian stimulation syndrome, multiple pregnancy, ovarian granulosa cell tumor, adrenal hyperplasia (male), liver disease, etc. negative: Positive: Tips: When extracting activated carbon solution (DCC), it is necessary to ensure sufficient mixing of DCC solution to avoid the separation effect due to the inconsistency of DCC concentration. Normal value Urine: male: 5 ~ 25μg / 24h female: before ovulation: 4 ~ 25μg / 24h ovulation period: 28 ~ 100μg / 24h luteal phase: 22 ~ 80μg / 24h pregnancy: <45000μg / 24h menopause: <10μg / 24h; Serum: Male: 40-115 ng/L (40-115 pg/ml) Female: Cycle 1-10 days: 61-394 ng/L (61-394 pg/ml) Cycle 11-20 days: 122-437 ng/L (122~ 437 pg / ml) cycle 21-30 days: 156 ~ 350 ng / L (156 ~ 350pg / ml) prepubertal, menopause: ≤ 40ng / L (≤ 40pg / ml). Clinical significance (1) elevated ovarian excess stimulation syndrome, multiple pregnancy, ovarian granulosa cell tumor, adrenal hyperplasia (male), liver disease and so on. (2) reduce ovarian dysfunction, congenital ovarian hypoplasia (Turne syndrome), Sheen syndrome (postpartum pituitary dysfunction syndrome), Sinmond (Simon) syndrome, anorexia nervosa, fetal-placental insufficiency, etc. . Low results may be diseases: high ovarian granulosa cell tumors may be diseases: multiple pregnancy, Sheen syndrome, endocrine function hyperactivity (1) Both the label and the standard are prepared from anhydrous ethanol and are easily volatilized. The lid should be tightly packed and stored in a refrigerator at 4 °C. (2) Antiserum should be stored at 4 °C after dilution into mother liquor. Do not put -20 °C at low temperature to avoid repeated freezing and thawing and reduce antiserum titer. (3) Gelatin, activated carbon, dextran and other reagents should be placed in a cool dry place. (4) 0.1% GPS is easy to deteriorate in summer even at 4 °C. It is best not to have too much dosing at one time. If it is found that it is obviously turbid, odorous and other deterioration phenomena, it should be re-matched. (5) When performing B and F separation, because DCC needs to maintain a constant contact adsorption time for the sample, the number of batching tubes should not be too much, and the time should not be too long. The control is added within 3 to 5 minutes; After separation, add the second batch. (6) When the activated carbon solution (DCC) is taken up, it is necessary to ensure sufficient mixing of the DCC solution to avoid the separation effect due to the non-uniform DCC concentration. (7) CH2Cl2 for extraction must be re-steamed and used. Inspection process 1 ml of the plasma sample was extracted with 0.2 ml of 0.1 mol/L NaOH and 6 ml of CH 2 Cl 2 , and the CH 2 Cl 2 layer was washed twice with distilled water for 1 ml of distilled water each time. Aspirate two portions of the 2 ml extract into a small test tube, add 50 μl of each of 3H-estrone, and blow dry with compressed air in a 45 ° C water bath. 0.2 ml of each antiserum was added, shaken, and placed at 4 ° C overnight. Add 5 g/L GPS 0.1 ml the next day, and add 2.5 g/LDCC 0.5 ml in a 4 ° C water bath. After standing for 15 min, the pellet was centrifuged for 10 min (3000 r/min), and 0.4 ml of the supernatant was aspirated and transferred to 8 ml of scintillation fluid for counting. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

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