Amniotic cell culture

Amniocytes culture is performed by ultrasound-guided amniocentesis to extract amniotic fluid, and the epithelial cells containing fetal exfoliation in the amniotic fluid are taken out for culture, and the cells are analyzed for the analysis of fetal karyotype. Take the supine position after emptying the bladder. Abdominal disinfection should be extended to the periphery centering on the puncture point, and the radius should be no less than 10cm. Spread a sterile hole towel. The puncture site was partially anesthetized with 0.5% lidocaine infiltration. The 7th sterile waist pierced needle was inserted vertically. Basic Information Specialist classification: maternity check check classification: biochemical examination Applicable gender: whether women are fasting: not fasting Tips: Women who are less than 12 weeks pregnant and more than 20 weeks old. Normal value The karyotype of normal males is 44 autosomes plus 2 sex chromosomes X and Y, which are usually expressed in 46, XY. Normal women have the same autosomes as men, and the sex chromosomes are 2 XX, usually indicated by 46, XX. 46 denotes the total number of chromosomes, and more than or less than 46 belong to an abnormal number of chromosomes. The missing sex chromosomes are usually represented by O. Clinical significance Abnormal results fetal chromosomal abnormalities. People who need to be examined give birth after the age of 35. They have had abnormal fetuses, repeated abortions, marriages in close relatives in the family, or family history of congenital diseases such as hemophilia and leukemia. It is usually done for prenatal evaluation at 16-18 weeks of gestation. Precautions Before the test: empty the bladder. When checking: Actively cooperate with the doctor. Inspection process Take the supine position after emptying the bladder. Abdominal disinfection should be extended to the periphery centering on the puncture point, and the radius should be no less than 10cm. Spread a sterile hole towel. The puncture site was partially anesthetized with 0.5% lidocaine infiltration. The 7th sterile waist pierced needle was inserted vertically. Through the two resistances of the abdominal wall and the uterine wall, when entering the amniotic cavity, there is a feeling of loss of tissue suddenly disappearing. When the needle core is pulled out, amniotic fluid is discharged, and about 20 ml of amniotic fluid is extracted with a syringe, and immediately sent for inspection as needed. Then, the puncture needle was removed, the pinhole was covered with a cotton ball and gauze, and the tape was fixed after pressing for 5 minutes. Not suitable for the crowd Inappropriate population: women with a pregnancy time of less than 12 weeks and greater than 20 weeks. Adverse reactions and risks Generally no complications occur.

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