Sperm acrosome activity

The acrosin is a neutral proteolytic enzyme (which acts like trypsin) present on the inner membrane of the sperm acrosome and on the equatorial membrane. It is an indispensable important proteolytic enzyme in the process of fertilization. The enzyme hydrolyzes the zona pellucida of the egg cell, allowing the sperm to pass through the cumulus and then through the zona pellucida, eventually merging with the egg. Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: not fasting Tips: You need to abstain from sex for 3-7 days before the test. It is recommended to avoid drinking and overwork in the near future. Normal value (36.72 ± 21.43) U / L (BAEE-ADH method). Clinical significance 1. Sperm acrosome enzyme activity is positively correlated with sperm severity and sperm integrity. 2. The reduction of sperm acrosome enzyme activity affects conception. Low results may be diseases: precautions for male infertility The enzyme hydrolyzes the zona pellucida of the egg cell, allowing the sperm to pass through the cumulus and then through the zona pellucida, eventually merging with the egg. Inspection process Take 0.25ml of liquefied semen in a plastic centrifuge tube, centrifuge at 500 × g for 5min; discard the supernatant, invert the centrifuge tube on the absorbent paper to remove the remaining solution, add 0.25ml 2% acetic acid solution, put the refrigerator at 4 ° C The mixture was extracted overnight (16-24 h), and then centrifuged at 1000×g for 10 min, and the supernatant was taken for use. NAD (1 mg/ml freshly prepared with pH 8.70.05 mol/LTris-HCl buffer was sequentially added to the cuvette. 0.1ml, BAEE1mg/ml0.5ml, ADH (3.46mg/ml) 0.1ml and Tris-HCl buffer 2.25ml, uniformly mixed, pre-warmed at 25 °C for 5min, then add 0.05ml acrosome enzyme extract immediately mixed Evenly, the change in absorbance of the reaction solution at 366 nm was recorded 10 minutes after the addition of the enzyme solution. Not suitable for the crowd There are no taboos. Adverse reactions and risks This check will not cause complications or harm.

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