Enzymatic tumor marker detection

Detection of enzyme tumor markers includes detection of prostatic acid phosphatase (PAP), neuron specific enolase (NSE), abnormal prothrombin (APT), α-L-fucosidase (AFU), and the like. PAP is important for the observation of the efficacy of prostate cancer, whether there is recurrence, metastasis and prognosis. NSE is a marker of neuroblastoma and small cell lung cancer. APT and AFU are significantly elevated in hepatocellular carcinoma. Basic Information Specialist classification: Oncology examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Included items: Cancer antigen 242 (CA242) Tips: Generally, the morning blood test is performed. Normal value The normal value is negative or less than a certain range. Clinical significance Abnormal results: Glutathione transferase (GST) is mainly found in liver, colon and gastric cancer tissues. Alkaline phosphatase (ALP, AKP) is different in sera of ovarian, pancreatic, gastric and breast cancer patients. The positive rate was 4%-33%, and the positive rate of ovarian cancer and seminoma was higher. Galactose transferase (GALT) has only GALT-I in normal human serum, and GALT-1 in patients with malignant tumors is only slightly higher than normal, and there is no statistical difference. The positive rate of GALT-2 in patients with malignant tumors can range from 73% to 83%. Most of these tumors are derived from endoderm, especially adenocarcinoma of the digestive tract or digestive gland, bronchial lung cancer and breast cancer. Lactate dehydrogenase (LDH) was used to detect the LDH level of gastric juice in patients with gastric cancer and atrophic gastritis. The results showed that there were significant differences between the two. It is believed that gastric LDH level can be used as a reference for the diagnosis of gastric cancer. People who need to be examined: Patients with related tumors need to be examined as a basis for diagnosis. Precautions Contraindications before the examination: Generally, the morning blood test is performed. Requirements for inspection: pay attention to serum is not contaminated, resulting in false positives, should be promptly sent for inspection. Inspection process Enzyme immunoassay: (1) Enzyme-linked immunosorbent assay (ELIFA) combines the advantages of both enzyme-linked immunosorbent assay (ELISA) and fluorescence immunoassay (FIA), and its sensitivity is significantly improved compared with traditional ELISA. The detected value is 10-15 mol/L. (2) Enhanced luminescent enzyme immunoassay (ELEIA) is a novel immunoassay developed in the late 1980s. It is characterized by enzymatic enhancement of luminescent signals and stable luminescence signals. The minimum detection value of this method is up to 10-20 mol / hole. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks No relevant information.

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