calmodulin

Calmodulin (CaM) is an important calmodulin protein, which is found in almost all eukaryotic cells. The receptor for calcium ions is involved in many functions of cells. The CaM content in the blood of various diseases changes. The determination of plasma caM concentration by radioimmunoassay is a meaningful indicator for the study of certain clinical diseases. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: An empty stomach is needed within 8 hours before blood draw. Normal value Plasma (623 ± 78.7) μg / L. Clinical significance Abnormal results: Increased calmodulin is found in diabetes, hypertension, and cancer. Need to check the crowd: Diabetes, hypertensive patients need to carry out this examination, the condition has been determined. High results may be diseases: hypothyroidism precautions Preparation before inspection: 1. An empty stomach is needed within 8 hours before blood draw. 2. You need to tell the doctor to take the medicine history before the examination. Attention during inspection: If you find any discomfort during the examination, you should inform the doctor promptly. Not suitable for the crowd: all suitable for the crowd. Inspection process Recombinant human CaM and rabbit anti-CaM were used to improve the solid-phase conditions of the antigen by ultraviolet irradiation treatment on the polystyrene reaction plate, and the conditions such as antigen coating solution and coating time were optimized to establish a simple, rapid and accurate condition. ELISA quantification technique. RESULTS: PB with pH 7.4 and 0.01 mol/L was used as coating solution. The coating and subsequent blocking time were 4 ° C, and the cells were allowed to stand for 72 h. The CaM coating concentration was 5 μg/ml, and the antigen-antibody reaction time was 37 ° C for 60 min. The best CaM quantitative results are obtained. Not suitable for the crowd No taboos. Adverse reactions and risks May cause an infection.

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