Secretory IgA in sputum

The SIgA in the sputum is secreted by the respiratory epithelial tissue and has the function of preventing the invasion of pathogenic microorganisms. When SIgA is reduced, the mucosal resistance is reduced, and it is prone to respiratory infection. After effective treatment, the immune function is improved, and SIgA can be recovered in the sputum. It has certain auxiliary diagnostic significance for acute bronchitis, acute exacerbation of chronic bronchitis and pneumonia. Basic Information Specialist classification: Respiratory examination classification: sputum examination Applicable gender: whether men and women apply fasting: fasting Tips: Antiserum should be treated strictly, there should be no interference ions, high speed centrifugation or 0.45μm ultrafiltration membrane filtration can be used to eliminate interference. Normal value 2.03 ± 0.21 mg / L. Clinical significance When SIgA is reduced, the mucosal resistance is reduced, and it is prone to respiratory infection. After effective treatment, the immune function is improved, and SIgA can be recovered in the sputum. It has certain auxiliary diagnostic significance for acute bronchitis, acute exacerbation of chronic bronchitis and pneumonia. Low results may be diseases: pediatric selective immunoglobulin A deficiency, bronchial pneumonia, viral pneumonia, acute tracheobronchitis in children, chronic bronchitis in children, bronchitis considerations 1. Effect of the ratio of antigen to antibody on turbidity: When the ratio of antigen to antibody is equal, the complex formed and the dissociation are equal. When the antigen content is too large, the formed complex is small and tends to be redissolved, and the reversible reaction is large. When the amount of the antibody is too large, the formed complex is bulky and insoluble, and the reversible reaction is small. Therefore, as a rate of turbidity, the antibody is excessive, which is favorable for the formation of the complex. 2. Quality impact of antiserum: Antiserum as an immune response requires high potency, high specificity and high purity. Antiserum for turbidity should also have: 1 The affinity of the antiserum is large, so the animal can be obtained by a small amount of long-term immunization. 2 The type of animal, the antisera prepared by R type animals (including sheep, guinea pigs, rabbits, etc.) have strong affinity, and the antigen and antibody are not easily dissociated after binding. 3 antiserum should be strictly treated, there should be no interference ions, high speed centrifugation or 0.45μm ultrafiltration membrane filtration can be used to eliminate interference. Long-term storage must be stored at -20 °C. 3. A solution for antigen-antibody reaction. 1 The antigen-antibody reaction has an optimum pH of 6.5 to 8.5. Above this limit, it is difficult to form a complex and may even be decomposed. 2 ionic strength can directly affect the binding of antigen-antibody reaction, the ionic strength of the complex formation is fast, and the low hinders the formation of the complex. The application of PBS is a better reaction solution. 4, the influence of hydrophilic agents: hydrophilic agents such as PEG (polyethylene glycol), Tween20, MES (emaphine-2 alkanesulfonic acid) can promote the formation of precipitation reaction. 5, the application of the standard curve: When the reagent conditions and room temperature conditions are unchanged, the curve can be used for a period of time, but not for a long time. The use of quality control serum calibration ensures accurate and reliable measurements. Inspection process Immediately after the collection of the sputum sample, the test was performed using the immuno-scattering turbidity operation method. Not suitable for the crowd Inappropriate people: Generally there are no people who are not suitable. Adverse reactions and risks No adverse reactions.

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