plasma free cortisol

Under normal circumstances, free cortisol content only accounts for less than 5% of the total, but it is this very small part of cortisol that plays a physiological role, plasma free cortisol is a biologically active part. Free cortisol is biologically active, can enter the cell, loses its activity in the liver, and can be filtered through the glomerulus into the urine. The free cortisol content in urine is directly proportional to the plasma free cortisol content. Reduced in adrenal insufficiency, Addison's disease, Simon's disease (Sinmond's disease), Xi Han's disease (sheehan's disease), congenital adrenal hyperplasia, long-term use of steroids. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduced in adrenal insufficiency, Addison's disease, Simon's disease (Sinmond's disease), Xi Han's disease (sheehan's disease), congenital adrenal hyperplasia, long-term use of steroids. Normal value: Morning (8:00): 166.5-441.4nmol/L Afternoon (16:00): 55.18-248.3nmol/L Midnight (0:00): 63.4-129.6 nmol/L Above normal: Increased in cortisol (Cushing's syndrome), ectopically produced ACTH tumors (oat type lung cancer, pancreatic cancer, thyroid cancer, parathyroid cancer, ovarian cancer, testicular cancer, colorectal cancer, gallbladder cancer, breast cancer) , mediastinal tumor, etc.), anterior pituitary hyperfunction. negative: Positive: Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value Radioimmunoassay (RIA method) Morning (8:00) 166.5 ~ 441.4nmol / L. In the afternoon (16:00) 55.18 ~ 248.3nmol / L. Midnight (0:00) 63.4 ~ 129.6nmol / L. Clinical significance 1, elevated in cortisol (Cushing's syndrome), ectopically produced ACTH tumors (oat-type lung cancer, pancreatic cancer, thyroid cancer, parathyroid cancer, ovarian cancer, testicular cancer, colorectal cancer, gallbladder cancer, Breast cancer, mediastinal tumor, etc.), anterior pituitary hyperfunction. 2, reduced in adrenal insufficiency, Edison disease, Simon disease (Sinmond disease), Xi Han disease (sheehan disease), congenital adrenal hyperplasia, long-term use of steroid hormones. Low results may be diseases: thyroid cancer, pancreatic cancer, high incidence of Xi'an syndrome may be diseases: Cushing's syndrome, congenital adrenal hyperplasia, adrenal insufficiency 1. Add 3H to each tube or the standard must be blown dry, then add the antibody, because 3H or standard is ethanol, otherwise it will damage the antibody. 2, the amount of 3H must be determined according to the experimental conditions (this article adds 7000 cpm), the less the amount of 3H antigen added in the corresponding range, the higher the sensitivity. Inspection process 1. Determination of plasma and urine samples: Take 0.1ml of plasma or one-tenth of 24h of mixed urine, all diluted to 0.5ml with distilled water, add 10ml of dichloromethane and 0.2ml of 0.1mol/L NaOH in 10ml extraction tube. After extraction for 5 min, the supernatant was discarded for a while, and the lower dichloromethane extract was washed twice with water, 1 ml each time, shaken for 1 min, centrifuged at 1500 r/min for 3 min, and the aqueous layer was discarded. The dichloromethane extract was taken in duplicates of 1 ml each. (complex tube), blow dry at 45 ° C compressed air, add 3H-cortisol 7000 cpm / 10 μl, blow dry at 45 ° C, add 0.2 ml antiserum application solution (diluted 1:3800 with 1g / L GPS), shake and set 4 °C overnight, the following steps are the same as the standard tube. 2, standard curve production: 0, 0.1, 0.3, 0.6, 1.0, 2.0, 4.0 ng (resection of each point), each tube plus 3H-cortisol 10ml, blow dry, add 0.2ml per antibody, take four Add 10 ml of 3H cortisol to each tube, add 1 g/L GPS0.7 and 0.2 ml, respectively, two tubes for the total pulse measuring tube, and the other two tubes for non-specific determination (BN%), shake well, and let 4 °C overnight. On the next day, add 5g/L GPS 0.1ml to each tube and shake it, then add 2.5g/LDCC0.5ml (the total tube T is not added). The free and conjugate were separated; shaken at 4 ° C for 10 min, centrifuged at 3000 r / min for 10 min to absorb 0.4 ml of the supernatant, added to 8 ml of scintillation fluid, mixed for 1 min, protected from light for 4 h, and measured with a liquid scintillation counter. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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