gastric lactate dehydrogenase isoenzyme

Lactate dehydrogenase has five isoenzyme forms, namely LDH1, LDH2, LDH3, LDH4, and LDH5, which can be separated by electrophoresis. The human heart, kidney and red blood cells are most LDH1 and LDH2. Liver and striated muscles are dominated by LDH4 and LDH5. There are more LDH3 in the spleen, pancreas, thyroid and adrenal glands. Lactate dehydrogenase isoenzyme is one of the indicators for observing myocardial diseases, liver and gallbladder diseases, and the like. Basic Information Specialist classification: Digestive examination classification: physical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Rare. Normal value: Gastric juice lactate dehydrogenase isoenzyme: 36-253U Above normal: The LDH of gastric cancer is increased by 2 to 3 times, and LDH5>LDH4>LDH3>LDH2>LDH1. Atrophic gastritis (moderate or severe) with intestinal metaplasia LDH4>LDH5>LDH3>LDH2>LDH1. negative: Positive: Tips: specimens should not be hemolyzed. Normal value 36 ~ 253U. Clinical significance 1, gastric cancer LDH increased 2 to 3 times, LDH5> LDH4> LDH3> LDH2> LDH1. 2, atrophic gastritis (moderate or severe) with intestinal metaplasia LDH4> LDH5> LDH3> LDH2> LDH1. High results may be diseases: gastric cancer, atrophic gastritis precautions 1, with serum LDH. 2, the specimen should not be hemolysis. Inspection process 1. Preparation of gel slide: Take a tube of 5g/L buffered agarose gel and heat it in a boiling water bath. Pipette 1.2ml of melted gel solution and spread it evenly on the cleaned 7.5cm×2.5cm slide. After cooling and solidification, dig the groove at the cathode end of the gel plate at about 1~1.5cm, and use the filter paper to remove the water from the tank. . 2. Loading: Add about 40 μl of serum to the tank with a micro-injector. 3, electrophoresis: voltage 75 ~ 100V, current 8 ~ 10mA / piece, electrophoresis 30 ~ 40min, to be serum albumin part of the movement 3 ~ 4cm. 4. Color development: 5 to 10 minutes before the end of electrophoresis, the 8g/L buffered agarose gel melted by the substrate color developing solution and the boiling water bath is mixed at a ratio of 4:5 to prepare a color developing gel solution, and the heat is set. Standby in water (about 50 ° C) (note that it is protected from light). After the electrophoresis was stopped, the gel slide was removed and placed in an aluminum box. Immediately, about 1.2 ml of the chromogenic gel solution was aspirated with a dropper, and rapidly dropped onto the gel slide to completely spread the coating, and the gel was solidified. After that, it was covered with light and placed in a 37 ° C water bath to keep the aluminum box floating on the water for 1 h. 5, fixed and rinse: take the color of the gel slide, immersed in the fixed rinse solution for 20 ~ 40min, until the background is no yellow, and then moved to distilled water rinsed several times, each time 10 ~ 15min. Not suitable for the crowd Patients with gastric perforation. Adverse reactions and risks Mucosal injury: Avoid excessive exertion when removing gastric juice from the gastric tube to avoid damage to the nasal cavity and esophageal mucosa.

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