Red blood cell reduced glutathione (GSH) and stability test

When glucose-6-phosphate glucose deoxygenase (G-6-PD) is deficient, the production of coenzyme NADPH, which is a glutathione reductase, is insufficient, so that GSH production of red blood cells is reduced, stability is lowered, and the amount of glutathione is measured. Can indirectly reflect the amount of G-6-PD. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: After taking blood, you need to press it at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. Normal value After the normal human red blood cells and acetophenone were kept warm, the GSH content decreased, generally not more than 20%. Clinical significance Normal human red blood cells added oxidative drug acetophenone, after incubation, did not affect its GSH content, but G-6-PD deficiency, GSH content decreased significantly. Red blood cell G-6-PD deficiency was significantly reduced, and general female heterozygotes decreased by about 50%; male patients were significantly reduced, up to 90%. Sometimes it is not even detected. Precautions Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The examination process: blood is drawn, blood is taken for examination, and venous blood is generally taken, and blood is drawn by a doctor or a nurse. The amount of blood drawn is determined according to the difference of the test content and the number of items. The blood volume is usually 2-20 ml, and the maximum is not more than 50 ml. Then the amount of glutathione is measured by a doctor. Not suitable for the crowd no. Adverse reactions and risks no.

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