Vibrio cholerae detection

The conventional method of Vibrio cholerae inspection is direct smear, staining inspection, and dynamic observation braking test. Clinical analysis of positive results can be used as a reference for rapid diagnosis. Gram staining showed typical Gram-negative Vibrio. The dark-field examination of the hanging drops shows a shuttle-like live movement. Positive immunological brake test has important reference significance. The above results can only be used for initial diagnosis, and the final diagnosis still needs to be based on the culture results. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Vibrio cholerae was not detected. Positive: Prompt for Vibrio cholerae infection. Tips: If you need to detect fecal occult blood by chemical method, please avoid eating red meat, liver and spinach, cabbage, broccoli and other foods three days before collection to avoid false positive results. Normal value Vibrio cholerae was not detected. Clinical significance Abnormal results: 1. Clinical analysis of positive results Microscopy can be used as a reference for rapid diagnosis. Gram staining showed typical Gram-negative Vibrio. The dark-field examination of the hanging drops shows a shuttle-like live movement. Positive immunological brake test has important reference significance. The above results can only be used for initial diagnosis, and the final diagnosis still needs to be based on the culture results. 2, positive in cholera. People who need to be tested: Frequent acute diarrhea, vomiting and other suspected cholera patients. Positive results may be diseases: cholera, paracholera precautions When checking: 1. Please avoid digging the part of the toilet urine and tap water when collecting; do not place the feces directly on toilet paper or paper towel. 2. To avoid interference with the test results, do not use cotton swabs to dig. 3. Do not collect too much feces to avoid having enough specimens for inspection. 4. If you need to detect fecal occult blood by chemical method, please avoid eating red meat, liver and spinach, cabbage, broccoli and other foods three days before collection to avoid false positive results. 5. If you use the immunological method to detect fecal occult blood, you do not need to limit the type of diet. 6. Because infants and young children are not easy to get enough samples at one time, if they need to be collected separately, please temporarily store the samples in the refrigerator to avoid bacterial growth. Not suitable for people: People without cholera symptoms. Inspection process 1. Enrichment A sample of 25 g was weighed and added to a jar containing 225 mL of alkaline peptone water (APW). Solid samples should be broken up with a homogenizer from 9000r/min to 10000r/min or fully shredded with scissors. (36±1) °C culture for 6h~8h and 16h~24h. For oyster samples, another sample should be prepared and placed in APW, cultured at 42 °C for 6h~8h and 16h~24h. 2, separation The surface growth of the enrichment medium of 6h~8h and 16h~24h was inoculated with 3mm~5mm, and at least one TCBS agar plate was finally streaked and cultured at (36±1) °C for 18~24h. On TCBS agar, typical Vibrio cholerae colonies are large, smooth, yellow, slightly flat, with opaque centers and translucent edges. 3. Initial identification (1) Two to five suspicious colonies on TCBS agar plates were picked with an inoculating loop, streaked on T1N1 agar plates, and cultured at (36 ± 1) °C for 12 h to 18 h. (2) The T1N1 agar surface culture was taken up with a sterile white filter paper, and an oxidase test was carried out by adding an oxidase reagent. (3) A sterile loop oxidase-positive culture was applied to a 0.5% sodium deoxycholate reagent drop to perform a sticky wire test. (4) The cultures in which the oxidase and the sticky silk test were positive by inoculation were inoculated, TSI, KIA and AGS were inoculated, and the bottom layer was punctured and the slope was scribed. (5) Inoculate T1N0 and T1N3 broth with the same culture as the above inoculation needle. (6) TSI, KIA, AGS, T1N0 and T1N3 broth were cultured at (36 ± 1) °C for 18h~24h. Not suitable for the crowd Not suitable for people: people without cholera symptoms. Adverse reactions and risks Generally no complications and harm.

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